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. 2019 Oct 14;1(1):fcz024. doi: 10.1093/braincomms/fcz024

Figure 1.

Figure 1

Scheme for the gene editing of patient-derived PSEN1 int4del iPSCs. (A) Strategy for the generation of isogenic cells using CRISPR/Cas9-editing of an iPSC line from an individual carrying the PSEN1 int4del mutation. (B) Genomic positioning of the editing site, showing ssODN repair arm (purple), mutation site (purple) and sgRNA (green) with PAM site (red). sgRNA (single guide RNA, for CRISPR/Cas9 targeting); RFLP (used for screening); ssODN (single-stranded oligodeoxynucleotide, for homology-directed repair).