Table 1.
Primers used in this study
| Assay | Forward | Reverse | |
|---|---|---|---|
| PSEN1 int4del specific | PCR (gDNA) | CGGAAGGATGGGCAGCTTACA | AGCCACGCAGTCCATTCAGG |
| PSEN1 exon 4 splicing | PCR (cDNA) | TGAGGACAACCACCTGAGCAA | TGGCAGCATTCAGAATTGAGT |
| PSEN1 | Sequencing/RFLP | AGGTCTAACCGTTACCTTGATTC | CAGCCCTATCCAGTAATACCATAC |
| ssODN | CRISPR/Cas9 | T*C*TCTGCATGGTGGTGGTCGTGGCTACCATTAAGTCAGTCAGCTTTTATACCCGGAAGGATGGGCAGCTGTACGTATGAGTTTTGTTTTATTA*T*T | |
| sgRNA | CRISPR/Cas9 | AGCTTTTATACCCGGAAGGA | |
| Off-target 1 | Sequencing | CACAGCGTTCACGTTGTATTG | CCAGGGAAGAAACAGAGACTAAC |
| Off-target 2 | Sequencing | ACAAGTAGACTTCTAGGCTGAAAC | CATGACTTCCTGAGGAGAACAG |
| Off-target 3 | Sequencing | TAGGTAAACACTGGCTGGAAAG | AGCAAGTGGGAAAGAAGACC |
| Off-target 4 | Sequencing | GCCTGCGATTTGAGGGATAAC | AGTTAAAGGGAGCAGGGACTAC |
| Off-target 5 | Sequencing | GAATCCTCCAGCCGGTCTTC | CAGCCCTGTCCCACCTTTC |
| RPL18a | qPCR | CCCACAACATGTACCGGGAA | TCTTGGAGTCGTGGAACTGC |
| APP | qPCR | GGTACCCACTGATGGTAAT | GGTAGACTTCTTGGCAATAC |
| PSEN1 | qPCR | TATCAAGTACCTCCCTGAAT | ACCATTGTTGAGGAGTAAAT |
| PSEN2 | qPCR | ATCTCTGTGTATGATCTCGT | TCCCCAAAACTGTCATAG |
| BACE1 | qPCR | GTCTCTGGTATACACCCATC | CATAGTTGTACTCCTTGCAG |
| TUBB3 | qPCR | CATGGACAGTGTCCGCTCAG | CAGGCAGTCGCAGTTTTCAC |
| TBR1 | qPCR | AGCAGCAAGATCAAAAGTGAGC | ATCCACAGACCCCCTCACTAG |
| CTIP2 | qPCR | CTCCGAGCTCAGGAAAGTGTC | TCATCTTTACCTGCAATGTTCTCC |
| ECE1 | qPCR | AGTGACACAGAAAACAACCT | GAACTGCAGTGTAGTCATTAAA |
| ACE | qPCR | GAAGTTTGATGTGAACCAGT | ACAGGATCTTGTTGTACTCCT |
| IDE | qPCR | CAAAGACTCACTCAACGAG | CTGAAAGATACATCCCATAG |
| NEP | qPCR | GAGGGGTCACGATTTTAG | AAGTCTGTACAAGGCTCAGT |
*
Phosphorothioate modification.