Fig. 6.

Specificity of FN3_hPD-L1-01 binder for ex vivo detection of hPD-L1 in tumors. (A) CT26/hPD-L1 tumor section stained with 1.25 nM of the FN3_hPD-L1-01 binder and Alexa Fluor 647^® 6XHisTag antibody (membrane staining). (B) CT26/hPD-L1 tumor sections stained with 0.3 nM of the FN3_hPD-L1-01 binder and Alexa Fluor 647^® 6XHisTag antibody. (C) Raji tumor section stained with 1.25 nM of the FN3_hPD-L1-01 binder and Alexa Fluor 647^® 6XHisTag antibody. (D) Raji tumor section stained with 0.3 nM of the FN3_hPD-L1-01 binder and Alexa Fluor 647^® 6XHisTag antibody. All sections were stained for the nuclei using DAPI (nuclei staining, center). Image acquisition was performed at 60× using an intravital microscope. Scale bar = 10 μm.