Figure 3.

The 3′ Extension of Precursor U8 Is Required for Optimal U8 Biological Activity

(A) Schematic depicting the experimental design for transient ubiquitous expression of U8 small nucleolar RNA (snoRNA) variants and mKate2 mRNA. Bolus size is monitored through the use of a phenol red dye, and successful uptake of the microinjected solution into the animal pole is traced using red fluorescence.

(B) Representative brightfield and fluorescent images of the genotype, exogenous snoRNA, and transgenic or fluorescent protein is indicated, taken at 48 hpf. U8 denotes human sequence whereas U8-3 denotes zebrafish. White arrowheads denote hindbrain swelling, red arrowheads denote aberrant yolk extension, black asterisks denote rescued hindbrain, and red asterisks denote rescued yolk extension.

(C) Quantitation of embryo length for the genotypes and introduced snoRNAs indicated. uninj—uninjected. n = 4 embryos per genotype. Error bars indicate SD from the mean.