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. 2020 Jan 29;2020:2938258. doi: 10.1155/2020/2938258

Table 1.

List of antibodies investigated, dilutions and antigen retrieval methods applied in the immunohistochemical analysis.

Antibody Clonality Manufacturer Dilution Antigen retrieval
Cytokeratin Clone AE1/AE3 Invitrogen®, Thermo Fisher Scientific® 1/200 Water bath/20 min
Vimentin Clone V9 DAKO®, Agilent Technologies® 1/1200 Water bath/20 min
CD31 Clone JC70A DAKO®, Agilent Technologies® 1/200 Pepsine
Synaptophysin Clone SP11 Thermo Fisher Scientific® 1/100 Water bath/20 min
c-Kit Polyclonal DAKO®, Agilent Technologies® 1/450 Water bath/20 min
GFAP Polyclonal Millipore® 1/2000 Water bath/20 min