Table 2.
Ct, ΔCt, ΔΔCt and RQ values for all genes under study for P4 and P6. nd = nondefined.
| Target gene | P4 | P6 | |||||
|---|---|---|---|---|---|---|---|
| Ct average OM-MSCs | ΔCt OM-MSCs | Ct average OM-MSCs | ΔCt OM-MSCs | ΔΔCt | RQ | Regulation | |
| Nanog | 35.9 ± 0.9 | 9.7 | 38.2 ± 0.0 | 13.5 | 5.4 | 0.0 | ↓ |
| Oct4 | 35.3 ± 0.0 | 9.1 | 36.5 ± 0.0 | 11.9 | 4.3 | 0.1 | ↓ |
| Sox2 | 36.0 ± 0.0 | 9.8 | 36.0 ± 0.0 | 11.3 | 3.0 | 0.1 | ↓ |
| CD105 | 33.9 ± 0.0 | 7.7 | 34.5 ± 0.0 | 9.8 | 3.7 | 0.1 | ↓ |
| CD90 | 26.2 ± 0.2 | −1.5 | 26.4 ± 0.0 | 1.7 | 3.2 | 0.1 | ↓ |
| CD73 | 32.9 ± 0.3 | 6.7 | 32.9 ± 0.3 | 8.2 | 3.0 | 0.1 | ↓ |
| CD44 | 29.4 ± 0.1 | 3.2 | 28.1 ± 0.1 | 3.4 | 1.7 | 0.3 | ↓ |
| CD45 | nd | nd | nd | nd | nd | nd | nd |
| CD34 | nd | nd | nd | nd | nd | nd | nd |
| BSP | 40.6 ± 0.0 | 14.4 | 35.8 ± 0.0 | 11.1 | −1.8 | 3.5 | ↑ |
| Runx2 | 35.1 ± 0.6 | 8.9 | 31.8 ± 0.0 | 7.1 | −0.3 | 1.2 | N |
| Coll II | 36.8 ± 0.0 | 10.6 | 30.3 ± 0.3 | 5.7 | −3.4 | 10.5 | ↑ |
| Aggrecan | 36.6 ± 0.6 | 10.4 | 31.6 ± 0.4 | 7.0 | −1.9 | 3.8 | ↑ |
| Ap−2 | 33.8 ± 0.4 | 7.6 | 27.8 ± 0.1 | 3.2 | −2.9 | 7.5 | ↑ |
| AdipoQ | nd | nd | nd | nd | nd | nd | nd |
| NGF | 36.9 ± 0.7 | 10.7 | 31.7 ± 0.4 | 7.0 | −2.2 | 4.5 | ↑ |
| GDNF | 34.4 ± 1.7 | 8.2 | 33.0 ± 0.0 | 8.4 | 1.7 | 0.3 | ↓ |
| Tenomodulin | 35.0 ± 0.0 | 8.8 | 35.0 ± 0.0 | 10.4 | 3.0 | 0.1 | ↓ |
| Desmin | 36.2 ± 0.0 | 10.1 | 36.3 ± 0.1 | 11.6 | 3.1 | 0.1 | ↓ |