FIGURE 1.

Quantification of the RNA kinetic rates through RNA metabolic labeling coupled with srRNA-seq. (A) The key steps of the RNA life cycle, and the corresponding RNA kinetic rates: synthesis (k₁) of premature RNA, processing (k₂) of premature into mature RNA, and degradation (k₃) of mature transcripts. (B) Incorporation of the uridine analog 4sU into newly synthetized transcripts. (C) Pre-existing and nascent RNA purification and sequencing through srRNA-seq. (D) Quantification of premature (P), mature (M), and nascent (N) RNA from srRNA-seq reads. (E) RNA life cycle mathematical modeling and quantification of the RNA kinetic rates in the steady-state limit.