TABLE 1.

Comparing strengths and pitfalls of four software packages for m⁶A detection from Nanopore dRNA-seq data.

	EpiNano	ELIGOS	MINES	Nanocompore
Requires training dataset	Yes	No	Yes	No
Requires comparison condition	No	Yes (cDNA)	No	Yes
Limited to RACH motifs	Yes	No	Yes	No
Single nucleotide resolution	Yes	Yes	Yes	No
Isoform resolution	Yes	Yes	Yes	Yes
Single molecule resolution	No	No	No	Yes
Able to distinguish different modifications	Yes	No	Yes	Yes