Figure 1.

(A) Buffalo fetal fibroblast cells were isolated and cultured using the explant technique for 72 h, then continuously cultured for another 2 days after removing the minced tissues. Pictures were taken at 24, 48, and 72 h after culturing and again 48 h after removing the minced tissues. (B) Buffalo fetal fibroblast cells were cultured in the presence of different concentrations of puromycin, and pictures were taken after 48, 72, and 96 h. (C) The percentage of transfected cells as determined by mCherry expression was affected by voltage. Cell survival was also greatly affected by the voltage. (D) The percentage of cells effectively transfected with supercoiled vector and expressing mCherry, was affected by the number of pulses. Cell survival was also greatly affected by the pulse number. Scale bar = 50 μm.