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. 2020 Apr 23;7:199. doi: 10.3389/fvets.2020.00199

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Copyright © 2020 Zhao, Nie, Tang, He, Xiao, Pang, Liang, Lu and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) The RGS reporter system was used to determine the efficiencies of sgRNAs; the cleavage activities were evaluated by FACS and are presented as the ratio of GFP⁺/RFP⁺ cells to all transfected cells; (B) Histograms show the relative cleavage activity of different sgRNAs; (C) Schematic overview of the strategy used to target the Actb locus in buffalo fetal fibroblast cells; (D) Knock-in efficiencies were evaluated by FACS and are presented as the ratio of mCherry⁺ cells to all transfected cells. **P < 0.01, ns, no significant difference.