Table 1.

Ingredient (%) and composition of the experimental diets.

	NC/CCa	100 Cu	150 Cu	80 Zn	100 Zn
Corn, grain	60.01	60.01	60.01	60.01	60.01
Soybean meal −48%	34.15	34.15	34.15	34.15	34.15
Dical. phos.	1.58	1.58	1.58	1.58	1.58
Soybean oil	1.53	1.53	1.53	1.53	1.53
Limestone	1.17	1.17	1.17	1.17	1.17
Common salt	0.35	0.35	0.35	0.35	0.35
DL-met	0.29	0.29	0.29	0.29	0.29
Vitamin premixb	0.25	0.25	0.25	0.25	0.25
L-lysine-HCL	0.22	0.22	0.22	0.22	0.22
Mineral premixc	0.08	0.08	0.08	0.08	0.08
L-thr	0.07	0.07	0.07	0.07	0.07
Cr2O3	0.30	0.30	0.30	0.30	0.30
Copper sulfate, 25%	–	0.040	0.060	–	–
Zinc sulfate, 22.2%	–	–	–	0.036	0.045
Nutrients
ME, kcal/kg	3,010	3,010	3,010	3,010	3,010
Crude protein	21.25	21.25	21.25	21.25	21.25
Lys%	1.32	1.32	1.32	1.32	1.32
Met%	0.63	0.63	0.63	0.63	0.63
TSAA%	0.98	0.98	0.98	0.98	0.98
Thr%	0.86	0.86	0.86	0.86	0.86
Ca%	0.90	0.90	0.90	0.90	0.90
avP %	0.45	0.45	0.45	0.45	0.45
Analyzed composition
Cu, mg/kg	10.69	104.73	149.14	7.40	7.26
Zn, mg/kg	97.35	93.96	93.89	169.69	197.59

^aNon-challenged control (NC), Challenged control (CC), 100 ppm Cu (100 Cu), 150 ppm Cu (150 Cu), 80 ppm Zn (80 Zn), and 100 ppm Zn (100 Zn).

^bProvided per kg of DSM Vitamin premix: Vit. A 2,204,586 IU, Vit. D₃ 200,000 ICU, Vit. E 2,000 IU, Vit. B12 2 mg, Biotin 20 mg, Menadione 200 mg, Thiamine 400 mg, Riboflavin 800 mg, d-Pantothenic Acid 2,000 mg, Vit. B6 400 mg, Niacin 8,000 mg, Folic Acid 100 mg, and Choline 34,720 mg.

^cProvided per kg of Mineral premix: Ca 0.72 g, Mn 3.04 g, Zn 2.43 g, Mg 0.61 g, Fe 0.59 g, Cu 22.68 g, I 22.68 g, and Se 9.07 g.