FIGURE 5.

Maximal CO₂ production rate of strains carrying deletions in horizontally acquired genes. Strains carrying single deletions in different horizontally acquired genes and showing statistically significant differences (t-test, p < 0.05) with respect to the wild type phenotype are shown for region A (A) and region C (B). Small scale fermentations in 150 mL of SM300 medium were performed for each strain and CO₂ release was measured as weight loss every 24 h. The CO₂ release curves were fitted to a sigmoid non-linear regression and the first derivative was calculated to obtain the maximal CO₂ production rate (Vmax, peak of the curves). The average values of three biological replicates are shown.