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. 2020 Apr 17;8:345. doi: 10.3389/fbioe.2020.00345

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Copyright © 2020 Jimi, Jaguparov, Nurkesh, Sultankulov and Saparov.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Development of granulation tissue and collagenosis. (A) Representative images of granulation tissues (parenthesized in red) stained with MT in each group. (B) Thickness and collagenosis in the granulation tissues. The values were evaluated with a morphometrical method. Granulations in the groups with cryogel alone and cryogel plus GF/C were significantly thicker than in the group with no treatment. Whereas collagenosis (blue in MT staining) in the group with cryogel plus GF/C progressed significantly as compared to the group with no treatment. n = 5. Bar = 100 μm. Values: mean ± SE. *P < 0.05, **P < 0.01 compared to the no treatment group.