Figure 4.

Specific cell morphology and tumorigenicity of Ra compared with Pa, Pb, and Rb

A. Morphology of Pa, Pb, Ra, and Rb. Green indicates phalloidin-stained F-actin, and blue indicates the cell nucleus stained with DAPI. Bar = 10 μm. B. Soft agar assays (40 ×). Colonies were microscopically visualized. C. Quantification of colonies formed in each well. 3 wells per group (repeated twice). Data are shown as mean ± SEM. D. Growth curves of tumors after subcutaneous injection of Pa, Pb, Ra, and Rb cells into the armpits of each NOD/SCID male or female mouse (4–6 weeks old). Tumor formation was monitored weekly after transplantation. Two weeks later, tumors were detected only in the Ra-transplanted mice. Ra tumor was monitored for the continuous growth and was removed on day 25 due to the large tumor size. No tumors were formed in the mice transplanted with Pa, Pb, or Rb, even after 90 days. Values are presented as mean ± SEM (5 mice in each group).