Figure 3.

Loss of Cdk8 Resulted in Lethality, Seizure, and Lifespan Decrease in Flies

(A) Ubiquitous expression of Cdk8 RNAi caused lethality, and it can be rescued by co-expression of human reference CDK19, whereas it cannot be rescued by co-expression of CDK19 variants (n = 8 crosses per each genotype). Statistical analyses were performed via one-way ANOVA followed by a Tukey post-hoc test. Results are means ± SEM, ^∗∗∗p < 0.001.

(B) Neuronal expression of Cdk8 RNAi caused severe lethality, but glial expression of Cdk8 RNAi did not cause any lethality. Co-expression of human reference CDK19 rescued the lethality, whereas co-expression of CDK19 variants failed to rescue the lethality (n = 9 crosses per each genotype). Statistical analyses were performed via one-way ANOVA followed by a Tukey post-hoc test. Results are means ± SEM, ^∗∗∗p < 0.001; n.s., not significant.

(C) Flies that lost Cdk8 in neurons exhibit strong bang sensitivity, which can be fully rescued by expression of human reference CDK19, whereas they fail to be rescued by expression of the variants. Statistical analyses were performed via one-way ANOVA followed by a Tukey post-hoc test. Results are means ± SEM, ^∗∗∗p < 0.001; n.s., not significant.

(D) Lifespan of flies that co-express human reference CDK19 or the variants with Cdk8 RNAi (n > 50 per each genotype).