Pharmacological inhibition of CSF1R with GW2580 does not alter astrocyte reactivity. A and B, Representative DAB immunostaining images (20× magnification, scale bar 40 μm) for GFAP-positive cells (A) and total GFAP-positive cell counts (B) in the SN after MPTP and GW2580 treatment. C, Representative immunofluorescent images (40× magnification, scale bar 25 μm) for iNOS (green) in GFAP-positive cells (red) with DAPI stained nucleus (blue). All data are represented as mean ± SEM; n = 3–4 animals per group; ***P < .001, ns = not statistically significant