Key Resources Table

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies: Flow cytometry
Ter119 – biotin labeled	B D Bioscience	51-09082J
B220 - biotin labeled	B D Bioscience	51-09082J
B220 – PECy7	B D Bioscience	552772
Gr-1 - biotin labeled	B D Bioscience	51-09082J
Gr-1 – AF700	B D Bioscience	557979
Gr-1 - FITC	Biolegend	108419
Mac1 - biotin labeled	B D Bioscience	51-09082J
Mac1 – AF700	B D Bioscience	557960
Mac1 - FITC	B D Bioscience	553310
CD3e - biotin labeled	B D Bioscience	51-09082J
CD3e - APC	B D Bioscience	561826
CD3e - PE	B D Bioscience	553048
CD8 - APC	B D Bioscience	553035
CD8 - PE	B D Bioscience	553032
Streptavidin – V500	B D Bioscience	561419
Streptavidin – APCCy7	B D Bioscience	554063
Sca-1 - PECy7	B D Bioscience	558162
c-Kit – APCef780	Invitrogen	47-1171-82
c-Kit -PE	B D Bioscience	553355
CD48 – AF700	Biolegend	103426
CD48 - FITC	B D Bioscience	557484
CD48 – BV605	B D Bioscience	740353
CD150 - FITC	eBioscinece	11-1501-82
CD150 - PE	eBioscinece	12-1501-82
CD150 - APC	Invitrogen	17-1501-81
CD45.2 – percpcy5.5	B D Bioscience	552950
CD45.2 – AF700	B D Bioscience	560693
CD45.2 - FITC	B D Bioscience	553772
CD34 – ef450	eBioscinece	48-0341-82
CD16/32	Invitrogen	12-0161-82
CD45.1 - PE	B D Bioscience	553776
CD45.1 – BV605	B D Bioscience	563010
H2Kd - FITC	B D Bioscience	553565
H2Kd - PE	B D Bioscience	553566
CD45 – APCCy7	B D Bioscience	557659
Anti-Drp1	abcam	Ab56788
Mitotracker dyes: Flow cytometry
TMRE	Sigma- Aldrich	87917
MitoSox™ red	Invitrogen	M36008
Mitotracker™ green FM	Invitrogen	M7514
Antibodies: Microscopy / Imaging
tomm20	Abcam	ab186734
drp-1	BD transduction laboratories	611112
alpha tubulin	Abcam	ab18251
anti-rabbit – AF488	Invitrogen	A21206
anti-rabbit AF546	Life technologies	A11010
anti-mouse AF555	Life technologies	A21422
Chemicals
Polyinosinic:polycytidylic acid	GE health care	Tlrl-pic
Doxycycline	Sigma- Aldrich	D9891-25G
Sucrose	Sigma- Aldrich	501212905
Mdivi-1	Sigma- Aldrich	M0199
DMSO	Fisher Scientific	BP231-100
Rotenone	Sigma- Aldrich	R8875
Oligomycin	Sigma- Aldrich	75351
Ficol histopcque	Sigma- Aldrich	10831
Paraformaldehyde	Electron Microscopy Sciences	15713
Triton X-100	Sigma-Aldrich	X10-100ml
Bovine serum albumin	Roche	3117332001
SlowFade gold antifade mountant with DAPI	Invitrogen	S36939
Fetal bovine serum	Atlanta	S11550
Fetal bovine serum	Omega	FB-01
Diffquick staining	Siemens	B4132-A
Media:
Stemspan medium	Stem cell technology	9650
Hank’s buffered saline solution		21-020-CV
Iscove’s Modified Dulbeco’s medium		10-016-CV
Cytokines:
Murine stem cell factor	Peprotech	250-03
Murine thrombopoietin	Peprotech	315-14
Human granulocyte colony stimulating factor		GF05-20ug
Murine interleukin-3	Peprotech	213-13
Erythropoietin	Espogen	NDC55513-126-10
Experimental Models: Strains
C57BI/6	In house, CCHMC	n/a
B6.SJL-Ptprca (BoyJ)	In house, CCHMC	n/a
Transgenic R26-M2rtTA;Col1a1-tetO-H2B-GFP	Jackson laboratories	016836
B6;129S-403 Gt(ROSA)26Sortm1.1(CAG-COX8A/Dendra2)Dcc	Jackson laboratories	018397
Drp1flox/flox mice	Dr. Hiromi Sesaki laboratory	n/a
MxCre1 mice	In house, CCHMC	n/a
NOD/SCID gammac−/−	In house, CCHMC	n/a
Deposited Data.
RNA-seq data	This paper	GSE141472
Software and Algorithms
FlowJo	FlowJo™ Software	https://www.flowjo.com/
GraphPad Prism 5.0	GraphPad Software Inc.	http://www.graphpad.com/scientific-software/prism/
ImageJ	NIH	https://imagej.nih.gov/ij/
Imaris	Oxford Instruments	https://imaris.oxinst.com
AltAnalyze	Emig, et al	http://www.altanalyze.org
Toppgene	Chen et al	https://toppgene.cchmc.org
EnrichR	Chen et al; Kuleshov et al	https://amp.pharm.mssm.edu/Enrichr/