Figure 2.

Competition assays with dCas9–VQR. A, structures of competitor DNA probes. The probes bore 2-bp segments upstream of PAM sequences that were either noncomplementary (probes 1.1–1.4) or complementary (probes 1.5–1.8) to gRNA. The PAM sequences are highlighted in yellow, and 2-bp segments matching the spacer sequence are highlighted in blue. B and C, effect of competitor probes 1.1–1.4 (B) and 1.5–1.8 (C) that bore indicated PAM sequences on the kinetics of dCas9–VQR–gRNA binding to beacon. The concentrations of competitors were 200 and 40 nm in experiments shown in B and C, respectively.