Silencing OMA1 increases H2O2-induced cell death.
A, immunoblots of cell lysate of a stable clone expressing OMA1 shRNA in OPA1-v1 cells. H2O2-induced OPA1 cleavage is attenuated upon OMA1 knockdown (KD) in OPA1-v1 cells. B, quantification of L- and S-OPA1 bands in immunoblotting showing the percentage of the total. C, LDH assays for cell death analyses. Decreasing OPA1 cleavage by OMA1 silencing in OPA1-v1 cells increases H2O2-induced cell death. ***, p = 0.00036; **, p = 0.0012 (Student's t test, unpaired). Error bars, S.E. D, OCRs of OPA1-v1 and OMA1-KD/OPA1-v1 cells in OXPHOS conditions with and without H2O2. Error bars, S.E. E, Cellular respiratory control ratios show significantly lower respiration coupling in OMA1-KD/OPA1-v1 cells treated with H2O2 in OXPHOS conditions, compared with that of OPA1-v1 cells. *, p = 0.0149 (Student's t test, unpaired). Error bars, S.E.