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. 2020 Mar 24;295(19):6652–6664. doi: 10.1074/jbc.RA119.012179

Figure 7.

Figure 7.

Characterization of α-synuclein from multiple system atrophy brains following sucrose gradient centrifugation and identification of seed-competent species. A, Western blot analysis (anti-α-synuclein antibodies Syn-1 and pS129) following SDS-PAGE of brain lysates fractionated by sucrose gradient centrifugation. A representative blot using MSA5 is shown. B, Western blot analysis (antibody Syn-1) of Sarkosyl-insoluble brain lysates. C and D, densitometric analysis of fractionated brain lysates. E, Western blot analysis (anti-α-synuclein antibodies Syn-1 and pS129) following native-PAGE of brain lysates fractionated by sucrose gradient centrifugation. F, Western blot analysis following SDS-PAGE of HEK cells expressing human WT α-synuclein seeded with fractionated lysates. Triton X-100–soluble cell lysates were blotted with Syn-1 and anti-GAPDH antibodies. Triton X-100–insoluble fractions were blotted with pS129. G, densitometric analysis of Triton X-100–insoluble pS129 bands of seeded cells. Densitometric results are expressed as the mean ± S.D. (n = 3).