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. 2020 Apr 3;295(19):6532–6542. doi: 10.1074/jbc.RA119.011802

Figure 4.

Figure 4.

Differential signaling through pAkt/pErk is mediated by XIB4035-induced modulation of GFRa1. A, representative blots taken from separate experiments showing pAkt and pErk signaling of compounds Q1047, Q525, Q112, and Q508 with or without XIB4035 pretreatment. V, vehicle. G, positive control GDNF. B, quantification of pErk signals of compounds Q1047, Q525, Q112, and Q508 in combination with the GFRa1 modulator XIB4035. XIB4035 pretreatment did not affect pErk signals induced by Q112 and Q508. However, Q1047 and Q525 signals were significantly increased. The data are expressed as means ± S.D. from five experiments standardized to compound alone, which was set at 100%. *, p < 0.05; ***, p < 0.0005, Bonferroni-corrected t test. C, quantification of pAkt signals of compounds Q1047, Q525, Q112, and Q508 in combination with the GFRa1 modulator XIB4035. XIB4035 pretreatment did not affect pAkt signals induced by Q1047 and Q525. However, Q112 and Q508 signals were significantly decreased. The data are expressed as means ± S.D. from five experiments standardized to compound alone, which was set at 100%. *, p < 0.05; **, p < 0.005, Bonferroni-corrected t test.