CCL2 promotes phosphorylation of GEFs and GAPs.
A–J, phosphorylation time courses in CCR2-expressing cells for selected GAPs and GEFs, including multiple phosphopeptides for RASAL2 (A–E) as well as ARHGEFs (F–H), GAPVD1 (I), and SLIT-ROBO Rho GTPase-activating protein 2 (SRGAP2) (J). Data represent CCL2-dependent changes in phosphopeptide intensity relative to unstimulated cells, mean ± S.E. (error bars) from three biological replicates.