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Incorporation of an oxygen atom into the hydroxylated product during oxidation of HE in the presence of O2˙̄. a, chemical structures of the products. b, online mass spectra of the products. c, LC-MS/MS traces of 2-OH-E+ containing 16O (left panel) or 18O (right panel). LC-MS/MS analyses were performed after incubation (30 min) of HE (20 μm) alone (control) or with 16O2˙̄ or 18O2˙̄ (0.2 μm O2˙̄/min, generated from HX/XO and 16O2 or 18O2, respectively).
