Table 3.

ΔG values of glycolytic enzymes in cells with or without PGK1 knockdown calculated according to the data in Table 1

	Enzyme	HK	PGI	PFK	Aldolase	TPI	GAPDH and PGK	PGAM	Enolase	PKM	LDH
	Keq	850	0.5	310	0.000064	0.05	158.00	0.17	0.48	363000.00	26300.00
	ΔG0 (kJ/mol)	−16.7	1.7	−14.2	23.8	7.50	−12.50	4.40	1.70	−31.40	−25.10
HeLa-NC	Q	0.019	0.22	0.59	0.000100	0.07	257.26	0.31	1.14	10.75	897.00
	ΔG (kJ/mol)	−26.66	−2.62	−15.75	−0.94	0.73	1.28	2.70	1.50	−26.33	−6.10
HeLa-siPGK1	Q	0.020	0.25	0.91	0.000180	0.07	135.08	0.24	1.53	6.47	1448.57
	ΔG (kJ/mol)	−26.45	−2.24	−14.62	0.60	0.91	−0.38	2.08	2.25	−27.64	−4.87
MGC80-3-NC	Q	0.017	0.27	0.37	0.000100	0.08	195.38	0.56	1.51	11.43	635.56
	ΔG (kJ/mol)	−26.86	−2.08	−16.97	−0.90	1.25	0.57	4.21	2.22	−26.17	−6.99
MGC80-3-siPGK1	Q	0.020	0.26	0.92	0.000200	0.08	67.93	0.56	1.88	7.63	891.43
	ΔG (kJ/mol)	−26.44	−2.21	−14.61	0.88	1.25	−2.15	4.21	2.78	−27.21	−6.12
RKO-NC	Q	0.035	0.35	1.98	0.000055	0.09	185.85	0.12	0.91	15.87	348.21
	ΔG (kJ/mol)	−25.05	−1.37	−12.63	−2.50	1.53	0.45	0.24	0.91	−25.32	−8.54
RKO-siPGK1	Q	0.033	0.45	2.26	0.000112	0.09	85.59	0.20	0.58	16.65	390.00
	ΔG (kJ/mol)	−25.22	−0.76	−12.29	−0.64	1.62	−1.55	1.53	−0.26	−25.20	−8.25
SK-HEP-1-NC	Q	0.006	0.27	0.25	0.000103	0.08	1126.12	0.27	1.77	26.83	382.35
	ΔG (kJ/mol)	−29.52	−2.08	−17.92	−0.86	1.24	5.09	2.29	2.63	−23.97	−8.30
SK-HEP-1-siPGK1	Q	0.008	0.31	0.28	0.000135	0.08	861.95	0.24	2.23	22.51	351.76
	ΔG (kJ/mol)	−28.84	−1.68	−17.70	−0.16	1.26	4.40	2.04	3.23	−24.42	−8.51
A549-NC	Q	0.004	0.18	0.29	0.000042	0.08	1146.11	0.32	1.18	97.52	231.56
	ΔG (kJ/mol)	−30.71	−3.10	−17.61	−3.18	1.02	5.13	2.79	1.59	−20.65	−9.59
A549-siPGK1	Q	0.004	0.28	0.32	0.000113	0.08	529.91	0.33	1.23	91.18	224.64
	ΔG (kJ/mol)	−30.73	−1.95	−17.30	−0.62	1.11	3.15	2.84	1.70	−20.82	−9.67