Skip to main content
. 2020 Mar 5;295(19):6263–6277. doi: 10.1074/jbc.RA119.012376

Figure 1.

Figure 1.

Genotoxic chemotherapy alters TNBC metabolism. A, viability measured by propidium iodide uptake following 24 or 48 h of exposure to chemotherapy agents. Selected concentrations (2.5 μm cisplatin, 0.5 μm doxorubicin) denoted by dashed lines. nonlinear curve fit by four parameter logistic regression. B, representative immunoblot of phospho–Ser-139 histone H2A.X (p-H2A.X) following exposure to chemotherapy agents at times and doses used for metabolite measurements. C, -fold change metabolite abundance over 24 h vehicle control for 186 metabolites measured by LC-MS/S in MDA-MB-468 and SUM-159PT cells treated with 2.5 μm cisplatin (CDDP) or 0.5 μm doxorubicin (DOXO).