Figure 10.
Active site of glycine-soaked crystals of H767A PlGoxA (PDB code 6VMV). A, omit Fo − Fc electron density contoured at 4.0σ for the active site modeled as CTQ. B, 2Fo − Fc electron density contoured at 1.0σ and Fo − Fc density contoured at 4.0σ are shown for the active-site adduct modeled as a carbinolamine, or C, as the product Schiff-base. D, overlay of the H767A PlGoxA (cyan and yellow) and WT PlGoxA (gray) PlGoxA-active sites. Putative hydrogen bonds are shown as dotted lines with distances indicated in Å. The hydrogen bonds indicated are from the H767A structure. Residues Tyr-766′ and His-767′ are of the neighboring protomer.