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Substrate binding and steady-state kinetic analysis of F316Y PlGoxA (A and C) and F316A PlGoxA (B and D). A and B, changes in absorbance at 600 nm were monitored during titration with glycine. The lines are fits of the data to Equation 1. C and D, steady-state kinetics data were also fit to Equation 5. Each data point is the average of three replicates. When error bars are not evident, it is because they are too small to extend beyond the circle.
