Table 1.

Kinetic and binding parameters for PlGoxA and variants

PlGoxA variant	Steady-state kinetics			Glycine binding
	kcat	K0.5	h value	K0.5	h value
	s−1	μm		μm
WTa	6.0 ± 0.2	187 ± 13	1.8 ± 0.2	103 ± 3	3.7 ± 0.4
D678Eb	1.0 ± 0.02	151 ± 17	1.0	26 ± 0.1	3.9 ± 0.1
H583A	NRc			NR
H583C	NR			NR
F316Y	8.0 ± 0.2	333 ± 17	1.5 ± 0.1	70 ± 1.2	1.7 ± 0.03
F316A	3.1 ± 0.2	783 ± 115	1.2 ± 0.1	175 ± 9	1.4 ± 0.1
Y766F	8.5 ± 0.2	666 ± 33	1.2 ± 0.04	527 ± 13	1.8 ± 0.1
H767A	NDd

^a Data are from Refs. 4, 7.

^b Data are from Ref. 13.

^c NR means no reaction.

^d ND means too slow to be accurately determined.