Table 3.

Primers used to generate site-directed mutations

Changed bases are underlined.

Mutation	Primers
H583C	Forward: 5′-GGT GGT TTT TGC CCT GGC GTT-3′
	Reverse: 5′-AAC GCC AGG GCA AAA ACC ACC-3′
H583A	Forward: 5′-GGT GGT TTT GCC CCT GGC GTT-3′
	Reverse: 5′-AAC GCC AGG GGC AAA ACC ACC-3′
F316Y	Forward: 5′-AAT ACC GAC TAT GCA GAT AAC TCA AAC TGG-3′
	Reverse: 5′-CCA GTT TGA GTT ATC TGC ATA GTC GGT AAT-3′
F316A	Forward: 5′-TTA AAT ACC GAC GCT GCA GAT AAC TCA-3′
	Reverse: 5′-TGA GTT ATC TGC AGC GTC GGT ATT TAA-3′
Y766F	Forward: 5′-CTT GGG TTT GTT GGC TTT CAT GCC GAA GGG-3′
	Reverse: 5′-CCC TTC GGC ATG AAA GCC AAC AAA CCC AAG-3′
H767A	Forward: 5′-CTT GGG TTT GTT GGC TAT GCT GCC GAA GGG-3′
	Reverse: 5′-CCC TTC GGC AGC ATA GCC AAC AAA CCC AAG-3′