Figure 3.

Comparison of biotin–streptavidin and chemical roadblock efficiency. A, single-round in vitro transcription of DNA templates without and with terminal biotin–streptavidin, internal desthiobiotin–TEG, or internal amino linker stall sites in the presence of 500 μm NTPs. Solid lines between gel images denote gel splices. B, quantification of the gels shown in A. All data are from two independent replicates; representative gels are shown. Desthiobiotin–TEG and amino linker experiments were performed together, and unmodified and biotin–streptavidin controls were performed separately.