Figure 6.

Stability of FB9s-r (A) and FB9s-b in rat CSF (B) and the stability of FB9s-r (C) and FB9s-b (D) in serum medium. The degradation reaction for an aptamer in triplicate was kept at 37 °C in rat CSF (A and B) or fetal bovine serum-containing DMEM (C and D). PAGE was used to visualize the time course of the RNase-catalyzed degradation of an aptamer sample; the arrow on the left of a representative PAGE image represents the full-length aptamer. The digitized band intensity, which was the average of the three experiments, is plotted on the right, where the time at which 50% of the sample was degraded (t_½) was estimated to be ∼5 days for both FB9s-b and FB9s-r in rat CSF. In serum medium, the t_½ for both FB9s-b and FB9s-r was estimated to be ∼4 days.