Figure 1.

Generation of Sun3^−/− mice. A, diagram illustrating the CRISPR/Cas9 targeting strategy to generate Sun3 knockout mice. A thymine deoxyribotide was inserted in exon 4 between nucleotide positions 184 and 185, predicted to cause a frameshift at amino acid position 63 that introduces a premature stop codon at position 64 (p.Pro62Leufs*2). B, representative Sanger sequencing chromatograms showing the thymine deoxyribotide inserted between nucleotide positions 184 and 185, as indicated by the red arrow. C, Western blotting using the rabbit anti-SUN3 antibody confirms the lack of full-length SUN3 proteins in testis lysates from 8-week-old Sun3^−/− mice.