Table 2. Means ± standard error of parameters analyzed in fresh, cooled and frozen/thawed dog semen, as expressed as percentages of total sperm cells. Membrane Fluidity and Permeability (YP/M540), Phosphatidylserine Translocation (AN/PI) and Membrane Integrity (FITC/PSA/PI).
| Parameter | Moment | ||
|---|---|---|---|
| YP-/M540- | 78.29 ± 6.22a | 75.76 ± 6.60a | 23.02 ± 9.12b |
| YP-/M540+ | 18.33 ± 5.54a | 22.55 ± 6.49a | 71.78 ± 9.85b |
| AN-/PI- | 79.90 ± 7.95a | 63.50 ± 4.66b | 27.19 ± 9.22c |
| AN+/PI- | 2.23 ± 2.06 | 6.88 ± 2.12 | 8.25 ± 10.16 |
| FITC-PSA-/PI- | 85.71 ± 6.22a | 70.37 ± 6.43a | 30.87 ± 9.90b |
Within a row, values with different superscripts differ significantly; P < 0.01 (n = 20 ejaculates). YP-/M540-, FITC-PSA-/PI-: cells displaying intact plasma and acrosomal membranes; YP-/M540+: cells with no permeability to YO PRO but increased fluidity (permeable to Merocyanine 540); AN-/PI-: no PS translocation; AN+/PI-: cells with PS translocation but not permeable to propidium iodide.