Figure 11.

DAB (3,3’-Diaminobenzidine) staining for detection of H₂O₂ in guard cell under control (A) and 300 mM NaCl (B–E). (B) control (zero ascorbic acid, AsA); (C) 150 mg L⁻¹ AsA; (D) 300 mg L⁻¹ AsA; E: 450 mg L⁻¹ AsA. Formation of brown precipitates showed the generation of H₂O₂ by guard cells. The borders of guard cells and stomatal pores were lined to make it more contrasting with the background. Raw images (without GC outlines) are provided in the Supplemental Figure S4. One (of 15) typical images is shown.