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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: Nat Protoc. 2020 Feb 24;15(4):1338–1370. doi: 10.1038/s41596-019-0261-4

Fig. 3 |. RNase treatment of cleared lysates.

Fig. 3 |

Examples of how to test the efficiency of the RNase treatment. Addition of an RNase mix induces the complete digestion of the two prominent ribosomal RNA species (18S and 28S), as illustrated by the absence of the corresponding bands/peaks. a, Cleared lysates of HeLa and HeLaS3 cells were processed with (+) or without (−) addition of an RNase mix. The respective total RNA samples were loaded onto a 1% agarose gel. b, Total RNA samples from HeLa S3 cells, untreated (Control) or treated with an RNase mix (RNase) were analyzed on a Bioanalyzer to monitor the length of the RNA fragments.