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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: Nat Protoc. 2020 Feb 24;15(4):1338–1370. doi: 10.1038/s41596-019-0261-4

Fig. 4 |. Comparison of gradients from different rotors.

Fig. 4 |

The use of different rotors (SW40 Ti and SW41 Ti) in similar conditions led to the same protein distribution through the 25 fractions of 5% to 50% sucrose density gradients. a, top panel: western blot analysis for RNA-dependent protein CTCF and RNA-independent protein ASNS in 25 fractions of representative control and RNase-treated samples after ultracentrifugation in a SW40 Ti rotor (30,000 rpm/114,000 g for 18 h at 4° C). Bottom panel: graph of the quantitative analysis of three replicates (N = 3) depicting the mean of three experiments with standard error of the mean (SEM). Adapted from Caudron-Herger et al., Mol Cell (2019) (ref. 24). b, Same as in a, with a SW41 Ti rotor (N = 1) (30,000 rpm/111,000 g for 18 h at 4° C). For all western blots, fractions 1 to 25 were loaded onto two membranes (1: fractions 1 to 13 and 2: fractions 14 to 25).