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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: Nat Protoc. 2020 Feb 24;15(4):1338–1370. doi: 10.1038/s41596-019-0261-4

Fig. 6 |. TMT workflow for sucrose gradient fractions.

Fig. 6 |

Proteins in the 25 sucrose density gradient fractions from control or RNase-treated samples were TCA precipitated and digested into peptides using trypsin. For each fraction, three biological replicates of the control sample and three biological replicates of the RNase-treated sample were labeled with individual tandem mass reporter tags (TMT, here TMT-6 plex), combined on a per-fraction basis and analyzed by LC-MS/MS. Fraction 1 is shown as an example here. The procedure is repeated for fractions 2 to 25.