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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: Nat Protoc. 2020 Feb 24;15(4):1338–1370. doi: 10.1038/s41596-019-0261-4

Fig. 7 |. Workflow of the statistical analysis.

Fig. 7 |

The pipeline is divided into seven parts, which include: a normalization step between the replicates and overall normalization (PART 1), a search for the starting parameters in order to fit all the distribution profiles using Gaussian curves (PART 2 and 3), a quality control of the fit with subsequent correction if needed (PART 4), the assessment of the p-value and adjusted p-values (adj. p-value, PART 5), and the evaluation of the shifts and subsequent selection of the significant shifts (PART 6). Lastly, a final table is computed and exported for further analysis (PART 7).