| Parameter | Value |
|---|---|
| Static modification | Carbamidomethyl (C) |
| +57.02146 Da | |
| TMT-6 (peptide N-term, K) | |
| +229.162932 Da | |
| Dynamic modification | Oxidation (M) |
| Enzyme | Trypsin |
| Maximum missed cleavages | 4 |
| Peptide mass tolerance (Da) | 1.00 |
| Peptide FDR (%) | 1 |
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