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. 2020 May 1;26:e921383-1–e921383-12. doi: 10.12659/MSM.921383

Figure 4.

Figure 4

miR-27b-3p inhibitor can partially reverse the effect of silencing HCP5 on cell viability and metastasis by modulating epithelial-mesenchymal transition (EMT)-associated markers. (A, B) Cell transfection efficiency was measured by qRT-PCR. GAPDH and U6 were used as internal controls. (C) Cell viability was detected by the Cell Couting Kit-8 assay. (D) Wound-healing assay was used to observe cell migration. (E) Transwell assay was used to observe cell invasion. (F) Colony formation assay was used to observe cell proliferation. (G, H) Western blot analysis was used to assess the expression of EMT-associated markers. GAPDH was used as an internal reference. All experiments were repeated 3 times and results were averaged. & p<0.05, && p<0.01, &&& p<0.001 vs. Silent Negative Control (siNC)+miR-27b-3p inhibitor Control (IC); # p<0.05, ## p<0.01, ### p<0.001 vs. siHCP5+IC; @ p<0.05, @@ p<0.01, @@@ p<0.001 vs. siNC+miR-27b-3p inhibitor (inhibitor).