Fig. 5.

ICG-001 inhibits proliferation of Merlin-negative meningioma cells partly by attenuating the FOXM1-mediated Wnt/β-catenin signaling. (A) Merlin and FOXM1 protein expression in the high-grade meningioma specimens with different background genotype. (B and C) Specimens with nuclear β-catenin staining showed significantly increased nuclear FOXM1 expression. Scale bars 100uM. (D) Increased Merlin expression reduced FOXM1 protein expression, whereas knockdown of Merlin expression led to upregulation of FOXM1 expression. (E) Knockdown FOXM1 in CH157-MN cells resulted in increased sensitivity to ICG001 treatment. (F) Schema Graph: Loss of Merlin stabilizes FOXM1 protein and subsequently enhances the Wnt/β-catenin pathway, resulting in an increased expression of Wnt downstream target genes and promoting meningioma development and progression.