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. 2020 Feb 3;2(1):vdaa011. doi: 10.1093/noajnl/vdaa011

Fig. 1.

Fig. 1

Delta24-RGD therapy significantly increases numbers of intratumoral ICOS+ and PD-1+ CD3+ T cells. C57BL/6J mice were inoculated i.c. with 5 × 104 GL261 cells and after 5 days treated i.c. with 2 × 107 pfu Delta24-RGD and control mice were treated with 10 µL PBS. Mice were sacrificed after 24 h, 48 h, 72 h, 7 days, 9 days, and 14 days post-therapy and cells were processed for flow cytometry (n = 3–5 mice/time-point/group). (A) Delta24-RGD significantly increases intratumoral CD3+ T cells on day 14 after therapy, reflecting an increase in CD8+ T cells. (B) Absolute numbers of CD3+ T cells were assessed and the expression of each co-signaling receptor within CD3+ T cells is analyzed. On day 14 after Delta24-RGD therapy, there was an increase in ICOS+ and PD-1+ TILs. P values were calculated with the Student t-test, *P < .05.