Figure 1.

Small protein gene identification in the E. coli genome over the past 6 years. (A) Histogram of currently annotated protein-coding genes in E. coli compared to those identified in 2013. (B) Histogram of currently annotated small protein genes in E. coli compared to those known in 2013. For both (A) and (B), the light gray bars represent small protein genes annotated in 2013, and the dark gray bars represent genes annotated in 2019. Data on annotated genes in 2013 are from E. coli K12 MG1655 genome annotation U00096.2. Data on annotated genes in 2019 were compiled from a combination of annotations from EcoCyc (92) and recent papers identifying new small proteins.