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. Author manuscript; available in PMC: 2021 Jan 31.
Published in final edited form as: Science. 2020 Jan 16;367(6477):580–586. doi: 10.1126/science.aay6018

Fig. 1. Mettl3 KO in mESCs leads to increased nascent RNA transcription and chromatin accessibility.

Fig. 1.

(A and B) Analysis of nascent RNA synthesis in WT or Mettl3−/− mESCs [(A), Mettl3−/−−1 and −2 are two independently generated KO lines], and Mettl3−/− mESCs rescued with WT or an inactive mutant Mettl3 (B). Nascent RNA synthesis was detected by using a click-it RNA Alexa fluor 488 imaging kit. EU, 5-ethynyl uridine; DAPI, 4′,6-diamidino-2-phenylindole. (C and D) Analysis of chromatin accessibility in WT or Mettl3−/− mESCs (C), and Mettl3−/− mESCs rescued with WT or mutant Mettl3 (D). DNase I–treated TUNEL assay was performed. For (A) to (D), the nucleus is counterstained by DAPI. EV (empty vector) refers to Mettl3−/− mESCs when transfected with empty vector plasmid. dsDNA, double-stranded DNA.