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. Author manuscript; available in PMC: 2020 May 11.
Published in final edited form as: J Vis Exp. 2020 Feb 22;(156):10.3791/60714. doi: 10.3791/60714

Figure 2: Calcein AM staining-based microscopic method for measuring NK cell-mediated cytotoxicity.

Figure 2:

(A) Schematic depicting the key steps of calcein AM staining-based microscopic method for measuring NK cell-mediated cytotoxicity. (B) NK cell-mediated cytotoxicity was analyzed in SK-HEP-1 cells expressing either NS shRNA or ATF4 shRNAs using the calcein AM method. Representative images are shown. Scale bar = 200 μm. (C) Percent of calcein AM-positive cells for the experiment presented in panel B. **p < 0.01.