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. Author manuscript; available in PMC: 2020 May 11.
Published in final edited form as: J Vis Exp. 2020 Feb 22;(156):10.3791/60714. doi: 10.3791/60714

Figure 3: FACS-based quantitative NK cell migration assay.

Figure 3:

(A) Schematic depicting the key steps of FACS-based NK cell migration assay. (B,C) NK cell migration assay was carried out after adding 50 ng of CCL2 to the bottom chamber of the 24 well plate. (B) Representative NK cell migration dot plots for control or CCL2-treated culture medium are shown. (C) NK cell migration data (mean ± SEM) is presented for the experiment shown in panel B. ***p < 0.001.