Table 1.

Pseudomonas aeruginosa P_pqsA-lux bioreporter assays of the compounds synthesized.

Example	R1	R2	R3	R4	R5	IC50 (μM) or Remaining Activity % (RA%) at 10 μM (bold)
						PAO1-L	PA14
7	H	4-Cl	H	H	H	0.98 ± 0.02	NA
8	Br	4-Cl	H	H	H	1.99 ± 0.23	1.60 ± 0.16
9	H	H	H	H	H	NA	NA
10	Br	H	H	H	H	NA	NA
11	H	3-Cl	H	H	H	RA% 34 ± 7.3	NA
12	Br	3-Cl	H	H	H	RA% 43 ± 20.2	NA
13	H	3,4-dichloro	H	H	H	NA	NA
14	Br	3,4-dichloro	H	H	H	3.1 ± 0.52	7.58 ± 0.82
15	H	4-F	H	H	H	NA	NA
16	Br	4-F	H	H	H	1.36 ± 0.21	NA
17	H	4-Me	H	H	H	1.86 ± 0.01	NA
18	Br	4-Me	H	H	H	RA% 32 ± 6.2	NA
19	H	4-OMe	H	H	H	NA	NA
20	Br	4-OMe	H	H	H	NA	NA
21	H	4-OCF3	H	H	H	RA% 29 ± 6	NA
22	Br	4-OCF3	H	H	H	RA% 23 ± 9	NA
23	H	4-(cyanomethyl)	H	H	H	0.62 ± 0.10	2 ± 0.17
24	H	4-phenoxy	H	H	H	0.38 ± 0.06	0.35 ± 0.06
25	Br	4-phenoxy	H	H	H	4.36 ± 0.42	NA
26	H	4-Br	H	H	H	1.71 ± 0.26	1.35 ± 0.19
27	Br	4-Br	H	H	H	23 ± 9	NA
28	H	4-(pyrimidin-2-yloxy)	H	H	H	1.04 ± 0.12	1.33 ± 0.33
38	H	2-Cl	H	H	H	NA	NA
39	Br	2-Cl	H	H	H	NA	NA
40	H	4-(pyridin-2-yloxy)	H	H	H	0.25 ± 0.12	0.34 ± 0.03
41	H	4-Cl	Me	H	H	NA	NA
42	H	4-Cl	H	Me	Me	NA	NA
43	H	4-Cl	H	Me	H	NA	NA
44	H	4-Cl	Me	H	Me	NA	NA
M64						0.32 ± 0.14	1.22 ± 0.34

Data shown are based on n = 9. NA, not active at 10 M concentration. Bold values represent percentage of remaining activities at 10 μM concentration.