KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Bacterial and Virus Strains
Biological Samples
Chemicals, Peptides, and Recombinant Proteins
20 uM Cas9-NLS	NEB	Cat #: M0646T
Alt-R CRISPR-Cas9 tracrRNA	IDT	Cat #: 1072532
Nuclease-Free Duplex Buffer	IDT	Cat # 11-01-03-01
Phenol Red	Sigma-Aldrich	Cat #: P0290
MS-222	Sigma-Aldrich	Cat #: E10521
Low-melt agarose	Bio-Rad	Cat #: 161-3111
Critical Commercial Assays
GeneJET Gel Extraction Kit	Thermo Scientific	Cat #: K0691
Deposited Data
Experimental Models: Cell Lines
Experimental Models: Organisms/Strains
Zebrafish: AB	ZIRC	ZFIN ID: ZDB-GENO-960809-7
Zebrafish: Tg(sp7:EGFP)b1212	ZIRC	ZFIN ID: ZDB-ALT-100402-1
Oligonucleotides
Protospacer sequence for crRNA design: plod2, AAGTATCCGTCTGTACGCAG	IDT	Alt-R® CRISPR-Cas9 crRNA, 2 nmol, custom design
Protospacer sequence for crRNA design: bmp1a, ATACGTGGGCCGCAGAGGAG	IDT	Alt-R® CRISPR-Cas9 crRNA, 2 nmol, custom design
Protospacer sequence for crRNA design: sp7:EGFP, GATGGCCGCGTCGATTCTGG	IDT	Alt-R® CRISPR-Cas9 crRNA, 2 nmol, custom design
sp7 F: ACCCCAAGAATCAAACCCCA sp7 R: AGTTAACATGCAGTAATCATCGCA	Sigma-Aldrich	Custom oligo
plod2 F: ACTGGAAAGCAGTGGACAGG plod2 R: AGGGTTGAAGACGGGGTAGA	Sigma-Aldrich	Custom oligo
bmp1a F: AGTTGGACGATATTTACCCTGGT bmp1a R: CGCACCCCCAGAGAAAACC	Sigma-Aldrich	Custom oligo
Recombinant DNA
Software and Algorithms
Fiji	Schindelin et al., 2012	https://imagej.net/Fiji
Prism 6 and 7	GraphPad	https://www.graphpad.com/
R	R Core Team, 2016	https://www.R-project.org/
FishCuT	Hur et al., 2017	https://github.com/elifesciences-publications/FishCuT
MATLAB	MathWorks	https://www.mathworks.com/products/matlab.html
Other