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. 2020 Mar 5;4:PO.19.00294. doi: 10.1200/PO.19.00294

FIG 4.

FIG 4.

Immune signaling activation in P2RY8–cytokine receptor–like factor 2 (CRLF2) fusion AML. (A) Immunohistochemical photomicrographs of the bone marrow biopsy showing phospho-specific staining of STAT5 and ERK, but not STAT3. (B) Immunohistochemical photomicrographs of the bone marrow biopsy showing staining of programmed death (PD)-1 in scattered lymphoid cells. PD-ligand 1 (PD-L1) staining was equivocal, whereas PD-L2 was negative. (C) Using multiplex immunofluorescence microscopy, with digital image analysis, we found that the patient’s bone marrow was highly enriched with CD8 plus granzyme B (GZMB) plus T cells. CD34+ staining highlights the myeloblast population at the time of AML transformation. We further identified a population of CD3+CD4+ T cells at baseline.