FIGURE 3.

miR-148b-5p and miR-744-3p are novel potential predictive biomarkers of acute rejection in lung transplantation (LTx) patients. A and B, ROC curves were generated to test the accuracy of the indicated microRNAs (miRNAs) in classifying patients (n = 26) according to presence of clinical acute rejection (c-AR) within the first y after LTx. The cutoff for miRNA expression was calculated using Youden’s J statistic. C and D, The ROC-generated cutoffs were used to sort LTx patients into LOW or HIGH expression groups for both miR-148b-5p and miR-744-3p and their ability to correctly classify LTx patients according to c-AR was analyzed using the enlarged cohort (n = 27). P values are from Fisher exact test. E–H, Freedom from c-AR (probability) was estimated using Kaplan-Meier curves in LTx patients within 1 y from transplantation (E and F) or during follow-up (G and H). Patients were sorted according to the receiver operating characteristic (ROC)-generated cutoff into high- and low-miRNA groups. The number of patients at risk is reported below the curves for all the groups. Indicated P values were computed by the log-rank test. AUC, area under the curve; CI, confidence interval; HR, hazard ratio.